Professor Jason Moffat
The Moffat Lab has broad interests in the regulation of cell growth and proliferation in cancer cell lines. We utilize genome-scale lentiviral-based RNA interference (RNAi) technology, CRISPR-Cas9-mediated gene editing and computational techniques to develop essential gene profiles in cultured mammalian cell lines and identify pairwise genetic interactions in order to define genetic interaction networks that are critical for cancer cell proliferation. Along with our collaborators, we are among a small group of labs in the world that is attempting to identify essential genes in human cancer cell lines. We are working on genes that encode cell surface proteins that are critical for cell proliferation and whose proper expression is dependent on metabolic pathways and cellular state.